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permeable inserts  (CELLTREAT Scientific)
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CELLTREAT Scientific permeable inserts
Permeable Inserts, supplied by CELLTREAT Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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temperature responsive cell culture inserts  (Cellseed Inc)
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Cellseed Inc temperature responsive cell culture inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Temperature Responsive Cell Culture Inserts, supplied by Cellseed Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transwell insert  (Greiner Bio)
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Greiner Bio transwell insert
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Transwell Insert, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nitrocellulose membranes  (Greiner Bio)
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Greiner Bio nitrocellulose membranes
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Nitrocellulose Membranes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptfe coated millicell cell culture inserts  (Merck & Co)
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Merck & Co ptfe coated millicell cell culture inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Ptfe Coated Millicell Cell Culture Inserts, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture inserts  (Nest Biotechnology)
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Nest Biotechnology cell culture inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Cell Culture Inserts, supplied by Nest Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture inserts - by Bioz Stars, 2026-06
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pores  (Greiner Bio)
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Greiner Bio pores
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Pores, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thincert cell culture membrane inserts  (Greiner Bio)
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Greiner Bio thincert cell culture membrane inserts
Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding <t>onto</t> <t>temperature-responsive</t> culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.
Thincert Cell Culture Membrane Inserts, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

Journal: Regenerative Therapy

Article Title: Characterization of the role and fate of nasal mucosal epithelial cell sheets in middle ear regeneration

doi: 10.1016/j.reth.2026.101130

Figure Lengend Snippet: Characterization and fabrication of nasal mucosal epithelial cell sheets derived from EGFP rats. (A) Schematic representation of the fabrication process for nasal mucosal epithelial cell sheets using EGFP rats. (B) Phase-contrast images 12 days after primary explant culture. (C) Phase-contrast micrographs of subcultured cells 7 days after seeding onto temperature-responsive culture dishes. (D) Macroscopic appearance of a cell sheet harvested by reducing the incubation temperature to 20 °C. (E) Colony-forming assay of cells harvested from the nasal mucosal epithelial cell sheet. (F) Fluorescence microscopy. Representative images of EGFP (green) and DAPI (blue) in the cell sheet. Scale bar = 50 μm. (G) Immunohistochemical staining for pan-cytokeratin. A representative cross-sectional image of the nasal mucosal epithelial cell sheet showing positive immunoreactivity for pan-cytokeratin (AE1/AE3). Scale bar = 25 μm.

Article Snippet: The cells were then seeded onto temperature-responsive cell culture inserts (CellSeed) at a density of 4 × 10 4 cells/cm 2 and cultured in KCM for 7 days.

Techniques: Derivative Assay, Incubation, Fluorescence, Microscopy, Immunohistochemical staining, Staining